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Beacon Designer™ outputs many TaqMan® probe-primer sets for the user to choose from.
TaqMan® Probes
Use Beacon Designer™ for optimal TaqMan® probes that help you study the differential gene expression or study SNP (single nucleotide polymorphisms) using TaqMan® SNP genotyping assays. You can also choose to design the increasingly popular Locked Nucleic Acid (LNA™) substituted TaqMan® probes, which are more stable than standard TaqMan® probes. To study DNA methylation, Beacon Designer™ helps design primers and TaqMan® probes for MethyLight assays. MethyLight is a high throughput assay used to discriminate between methylated and unmethylated DNA. Using Beacon Designer™, you can design suitable control primers and probes by designing oligos for the methylated and the unmethylated strands, and for the untreated DNA sequence. With any of these chemistries, you could evaluate a pre-designed/published primers or TaqMan® probes. The design results are reported in a ranked order, giving you a list of alternates to choose from. Depending on the oligo properties, a rating is assigned. Please read the Rating section in the online help for details.
Molecular Beacons
Design or evaluate standard molecular beacons or molecular beacons for NASBA® assays. NASBA® is a primer-dependent technology that can be used for the continuous amplification of nucleic acids in a single mixture at one temperature. You can now design molecular beacons for NASBA® assays for single template or multiplex reactions.
双标记探针的设计
Beacon Designer目前支持四种基于探针的化学物质:
TaqMan探针
使用Beacon Designer优化TaqMan探针,帮助您研究差异基因表达或使用TaqMan SNP基因型检验研究SNP(单核苷酸多态性)。您也可以选择设计越来越流行的锁定核酸(LNA)取代TaqMan探针,这些探针比标准TaqMan探针更稳定。为了研究DNA甲基化,Beacon Designer帮助设计引物和TaqMan探针进行甲基化分析。甲基光是一种高通量分析,用于区分甲基化和非甲基化DNA。使用Beacon Designer,通过设计甲基化和未甲基化链的寡核苷酸,以及未经处理的DNA序列,可以设计合适的控制引物和探针。有了这些化学物质,您可以评估预先设计的引物或TaqMan探针。设计结果按顺序排列,给您一个可供选择的替代品列表。根据oligo的属性分配等级。
Beacon Designer可以自动设计实时引物和探针。它被世界各地的分子生物学家用来设计成功的实时PCR分析。它节省了很多失败的实验所浪费的时间和金钱。Beacon Designer是实时引物和探头设计的灵活的解决方案。
可用的设计选项
SYBR Green试验设计
SYBR Green可能是实时PCR化学中常用的。Beacon Designer设计SYBR Green PCR引物,帮助您评估预先设计的SYBR Green PCR引物集。您可以完全控制设计参数,或者您可以使用默认值,在经过大量的研究和与的交流之后选择。您甚至可以为您的特定研究需求选择您自己的默认设计参数。
除了在感兴趣的基因上定位引物外,信标设计者还可以设计外显子或外显子内含子连接的引物。这些引物设计的外显子内含子连接有助于选择性帮助从gDNA选择性扩增cDNA。
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